Journal: BioTechniques

Article Title: Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples

doi: 10.2144/btn-2017-0102

Figure Lengend Snippet: Absorbance control values of the samples at 1 cm pathlength measured using a high accuracy dual beam spectrophometer at 22°C and 0.8 nm spectral bandwidth. The values in the parentheses are CV (%) of the mean from N = 9 (genomic DNA) and N = 4 (oligoncleotide samples), except for the *SRM 2082 components that are the standard uncertainties calculated as described in the NIST certificate of analysis ( https://www.nist.gov/srm ) calcuated using extensive measurements.

Article Snippet: Human genomic DNA (gDNA) was obtained from Coriell Institute for Medical Research (Camden, NJ).

Techniques: Control

Journal: BioTechniques

Article Title: Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples

doi: 10.2144/btn-2017-0102

Figure Lengend Snippet: Percentage of control values and coefficients of variation (% CV) using microvolume spectrophometers (MV Spec), and a microvolume (MV) plate reader. The values are the average of data acquired over three days as shown in , with the exception of MV Spec C, where the data were acquired in one day. The values in the parentheses are 1 CV (%) and the number (N) of measurements.

Article Snippet: Human genomic DNA (gDNA) was obtained from Coriell Institute for Medical Research (Camden, NJ).

Techniques: Control

Journal: BioTechniques

Article Title: Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples

doi: 10.2144/btn-2017-0102

Figure Lengend Snippet: Mass concentration values of human genomic DNA samples obtained using the different methods. Absorbance values and derived mass values of the human genomic DNA samples from a double-beam (DB) spectrophotometer, microliter volume (MV) spectrophotometers, microvolume (MV) plate reader, fluorescent dye binding, and digital PCR assays.

Article Snippet: Human genomic DNA (gDNA) was obtained from Coriell Institute for Medical Research (Camden, NJ).

Techniques: Concentration Assay, Derivative Assay, Spectrophotometry, Binding Assay, Digital PCR, Lambda DNA Preparation

Journal: BioTechniques

Article Title: Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples

doi: 10.2144/btn-2017-0102

Figure Lengend Snippet: Mass concentration of the human genomic DNA sample calculated from droplet digital PCR data with different assumptions of the droplet size.

Article Snippet: Human genomic DNA (gDNA) was obtained from Coriell Institute for Medical Research (Camden, NJ).

Techniques: Concentration Assay, Digital PCR

Journal:

Article Title: Efficient Approach to Unique Single-Nucleotide Polymorphism Discovery

doi:

Figure Lengend Snippet: The results of scanning sWXD3868 for SNPs by method 1 is shown in A. CEPH parents are 1, 2, 3, 4, and the CEPH population pool is 5 (additional detail about DNAs used are included in Methods). Sequencing was done with the dRhodamine terminators in A. The results for method 2 are shown in B. The CHM1 is sample 6 and the CEPH population pool is sample 5. Sequencing was done with the BigDye terminators in B. (↓) SNP locations. The small blue underhand for the T peak (↓) in B, sample 6 is a common sequencing artifact and was seen in a number of T peaks in this sequencing trace.

Article Snippet: Genomic DNA samples from 4 female CEPH parents (individual 1, CEPH K102-02, Coriell NA04479; individual 2, CEPH K1340-02, Coriell NA07019; individual 3, CEPH K1345-02, Coriell NA07348A; individual 4, CEPH K13294-02, Coriell NA07434 Coriell Institute; 8 chromosomes) and a pooled genomic DNA sample consisting of 40 female and 40 male CEPH parents (120 X chromosomes) were amplified by PCR.

Techniques: Sequencing

Journal:

Article Title: Efficient Approach to Unique Single-Nucleotide Polymorphism Discovery

doi:

Figure Lengend Snippet: The results of scanning sWXD3654 for SNPs by methods 1 and 2 are shown. (Method 1) CEPH parents are 1, 2, 3, and 4 and the CEPH population pool is 5 (additional detail about DNAs used is included in Methods); (method 2) CHM1 is sample 6, and the CEPH population pool is sample 5. The CEPH population pool is shown only once. Sequencing was done with the BigDye terminators. (↓) SNP locations.

Article Snippet: Genomic DNA samples from 4 female CEPH parents (individual 1, CEPH K102-02, Coriell NA04479; individual 2, CEPH K1340-02, Coriell NA07019; individual 3, CEPH K1345-02, Coriell NA07348A; individual 4, CEPH K13294-02, Coriell NA07434 Coriell Institute; 8 chromosomes) and a pooled genomic DNA sample consisting of 40 female and 40 male CEPH parents (120 X chromosomes) were amplified by PCR.

Techniques: Sequencing